In the article, the questions of the chronology of the Neolithic cultures in the Volga-Kama basin are discussed. This discussion is based on the significant series of radiocarbon dates obtained on the different organic materials in several last years. The dates were obtained as tradition scintillation technique, as well as AMS method. There was established the different age of cultural complexes on the same site and the time of transition from early to later Neolithic was determined in the Northern Cis-Caspian region. The beginning of the early Neolithic and the final of the later stage were clarified. The Neo-Eneolithic period in the Lower Volga region has been verified. The frameworks of the Early Neolithic and coexistence of Mesolithic and Neolithic societies in the forest-steppe zone of the Volga region were considered. The low border of the Neolithic period in the forest zone of the Middle Volga region was established. In the Cis-Kama region, the chronological frameworks of the Kamskaya culture were determined and the chronological ratio between complexes of unornamented, pricked and combed ornamented ceramics were explained.